DNA and General PCR Methods: Recombineering
نویسندگان
چکیده
منابع مشابه
Recombineering
Recombineering, a recently developed technique for efficient genetic manipulation of bacteria, is facilitated by phage-derived recombination proteins and has the advantage of using DNA substrates with short regions of homology. This system was first developed in E. coli but has since been adapted for use in other bacteria. It is now widely used in a number of different systems for a variety of ...
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The use of BAC plasmids for transgenic gene expression is also gaining popularity over traditional proximal promoter driven transgene expression because the BAC typically contain most, if not all, of the important regulatory elements required to recapitulate endogenous gene expression (Giraldo and Montoliu 2001). Maintaining the coding sequence within its regulatory locus insulates the transgen...
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a Wyss Institute for Biologically Inspired Engineering, Harvard University, 3 Blackfan Circle, 5th Floor, Boston, MA 02115, USA b Department of Systems Biology, Harvard Medical School, 200 Longwood Avenue, Alpert 536, Boston, MA 02115, USA c Systems and Synthetic Biology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands d Program in Biomedical Informatics, Harvard Med...
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Formation of higher-order structure of nucleic acids (hairpins or loops, for example) may impact not only gene regulation, but also molecular biology techniques and approaches critical for design and production of vectors needed for genetic engineering approaches. In the course of designing vectors aimed to modify the murine Foxd3 locus through homologous recombination in embryonic stem cells, ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2009
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000113214